Albumin antibody

Catalog No. ABIN118031

The Rabbit Polyclonal anti-Albumin antibody has been validated for EIA. It is suitable to detect Albumin in samples from Human.

Quick Overview for Albumin antibody (ABIN118031)

Target: Albumin (ALB)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Albumin antibody is un-conjugated

Application: Enzyme Immunoassay (EIA)

Product Details anti-Albumin Antibody

Specificity: Purification was by Protein A Sepharose Fast Flow followed by POROS EP antigen resin affinity purification. The eluate was dialyzed against PBS with sodium azide, concentrated by Amicon ultrafiltration, 0.2 μm filtered, aseptically filled, and stored at 2-8° C. An aliquot was submitted for final qualification. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum. Analysis by SDS-PAGE was used to determine that this preparation is substantially free of aggregates and shows a banding pattern consistent with purified Rabbit IgG.

Purification: Immunoaffinity Chromatography.

Immunogen: Human Serum Albumin.

Application Details

Application Notes: Suitable for most Immunological methods. Recommended Dilutions: ELISA: 1/20,000-1/100,000. Western Blot: 1/2,000-1/10,000. Immunohistochemistry: 1/1,000-1/5,000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.04 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 0.01 % (w/v) Sodium Azide as preservative without stabilizers.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Dilute only prior to immediate use

Storage: 4 °C/-20 °C

Storage Comment: This product is stable for one month at 2-8 °C as an undiluted liquid. For extended storage aliquot contents and freeze at -20 °C or below. Avoid cycles of freezing and thawing.

Target Details for Albumin

Target: Albumin (ALB)

Alternative Name: Albumin

Background: Albumin is a soluble, monomeric protein which comprises about one half of the blood serum protein. Albumin functions primarily as a carrier protein for steroids, fatty acids, and thyroid hormones and plays a role in stabilizing extracellular fluid volume. Mutations in this gene on chromosome 4 result in various anomalous proteins. Albumin is a globular unglycosylated serum protein of molecular weight 65,000. The human albumin gene is 16,961 nucleotides long from the putative 'cap' site to the first poly(A) addition site. It is split into 15 exons which are symmetrically placed within the 3 domains that are thought to have arisen by triplication of a single primordial domain. Albumin is synthesized in the liver as preproalbumin which has an N terminal peptide that is removed before the nascent protein is released from the rough endoplasmic reticulum. The product, proalbumin, is in turn cleaved in the Golgi vesicles to produce the secreted albumin.Synonyms: ALB, BSA, HSA, Serum Albumin

Gene ID: 213

NCBI Accession: NP_000468

UniProt: P02768

Pathways: Lipid Metabolism